14.14 Red cell folate (categories 3)

Red cell folate data available for STOP cohort only.


Over detection limit <14900 vs ≥14900.

14.14.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 314.979852 1 <0.0001 ***
rcfolate_gp3 2.701767 1 0.1 ns
GA_samp 91.008623 1 <0.0001 ***
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 33.967504 1 <0.0001 ***
rcfolate_gp3 2.501515 1 0.1 ns
GA_samp 1678.317810 1 <0.0001 ***
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 566.365724 1 <0.0001 ***
rcfolate_gp3 4.710131 1 0.03 *
GA_samp 779.739710 1 <0.0001 ***
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp3 1 Inf 1.930 1.930 0.2 ns
GA_samp 1 Inf 2.869 2.869 0.09 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp3 1 Inf 1.630 1.630 0.2 ns
GA_samp 1 Inf 2.215 2.215 0.1 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp3 1 Inf 2.315 2.315 0.1 ns
GA_samp 1 Inf 1.029 1.029 0.3 ns

14.14.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 18.05 (13.12, 24.83) <0.0001
rcfolate_gp3≥14900 1.07 (0.99, 1.16) 0.10
GA_samp 1.13 (1.10, 1.16) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.70 (1.42, 2.03) <0.0001
rcfolate_gp3≥14900 0.96 (0.92, 1.01) 0.11
GA_samp 1.36 (1.34, 1.39) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.06 (0.05, 0.07) <0.0001
rcfolate_gp3≥14900 0.93 (0.88, 0.99) 0.03
GA_samp 1.33 (1.30, 1.36) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 8.07 (5.51, 11.81) <0.0001
rcfolate_gp3≥14900 1.08 (0.97, 1.20) 0.16
GA_samp 1.03 (1.00, 1.06) 0.09
HOMA-IR
Var Effect P
(Intercept) 1.74 (1.17, 2.61) 0.007
rcfolate_gp3≥14900 1.08 (0.96, 1.20) 0.20
GA_samp 1.03 (0.99, 1.06) 0.14
Glucose (mmol/L)
Var Effect P
(Intercept) 4.87 (4.56, 5.21) <0.0001
rcfolate_gp3≥14900 1.02 (0.99, 1.04) 0.13
GA_samp 1.00 (0.99, 1.00) 0.31

14.14.3 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

14.14.4 Estimated marginal means

Outcome rcfolate_gp3 response SE df lower.CL upper.CL
PRL (ng/ml) <14900 73.126 5.474 69.360 62.983 84.903
PRL (ng/ml) ≥14900 78.147 5.839 68.978 67.326 90.709
hPL (μg/ml) <14900 59.412 1.867 67.936 55.800 63.258
hPL (μg/ml) ≥14900 57.204 1.797 67.858 53.728 60.904
GH2 (ng/ml) <14900 1.495 0.051 100.542 1.397 1.600
GH2 (ng/ml) ≥14900 1.396 0.048 100.355 1.304 1.493
Insulin (uU/ml) <14900 11.221 0.408 Inf 10.449 12.050
Insulin (uU/ml) ≥14900 12.091 0.474 Inf 11.196 13.057
HOMA-IR <14900 2.371 0.092 Inf 2.198 2.558
HOMA-IR ≥14900 2.549 0.105 Inf 2.351 2.764
Glucose (mmol/L) <14900 4.717 0.037 Inf 4.646 4.789
Glucose (mmol/L) ≥14900 4.802 0.043 Inf 4.720 4.886

14.14.5 Plots

14.14.6 Post-hoc comparisons

Outcome Contrast Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) ≥14900 / <14900 1.07 (0.99, 1.16) 0.1
hPL (μg/ml) ≥14900 / <14900 0.96 (0.92, 1.01) 0.1
GH2 (ng/ml) ≥14900 / <14900 0.93 (0.88, 0.99) 0.03
Insulin (uU/ml) ≥14900 / <14900 1.08 (0.97, 1.20) 0.2
HOMA-IR ≥14900 / <14900 1.08 (0.96, 1.20) 0.2
Glucose (mmol/L) ≥14900 / <14900 1.02 (0.99, 1.04) 0.1